Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Enhancing the structural stability of P29-targeted monoclonal antibodies via β-hydroxybutyrylation modification improves their therapeutic performance in alveolar echinococcosis

doi: 10.3389/fcimb.2025.1716047

Figure Lengend Snippet: Therapeutic efficacy of P29 mAb kbhb in a murine model of AE. (A) Longitudinal monitoring of body weight in PSC-infected mice. Body weights were recorded at the indicated timepoints (0, 3, and 5 months post-infection) in mice treated with IgG (n=9), P29 mAb (n=9), or P29 mAb kbhb (n=9). Treatment was initiated at 3 months post-infection. (B, C) Organ indices at study endpoint. Liver index (B) and spleen index (C) were measured in PSC-infected mice at 5 months post-infection following treatment with IgG (n=9), P29 mAb (n=9), or P29 mAb kbhb (n=9). (D-G) Parasitic burden assessment. (D) Histopathological analysis. Upper panel: HE staining showing structural features of alveolar hydatid cyst. Lower panel: PAS staining highlighting carbohydrate-rich layers. Arrows indicate: - Black: Key structures (GL = germinal layer, LL = laminated layer, PV = parasitic vesicle). - Red: Degenerative changes in the capsule wall. (E) PSC regenerative capacity. PSC numbers were quantified in alveolar hydatid cyst sections from AE model mice treated with IgG (n=9), P29 mAb (n=9), or P29 mAb kbhb (n=9). Three microscopic fields were analyzed per sample. (F) Representative histological images of TUNEL staining in alveolar hydatid cyst tissues. Cyst sections from AE model mice treated with IgG, P29 mAb, or P29 mAb kbhb are shown. For each treatment group, low-magnification views (left panels; scale bars = 200 μm) display overall tissue architecture and TUNEL + cell distribution, with boxed regions indicating the areas selected for high-magnification imaging (right panels; scale bars = 100 μm). The high-magnification images highlight detailed morphological features of apoptotic cells (magenta staining). (G) Quantitative analysis of TUNEL staining fluorescence intensity. Apoptotic levels were assessed in alveolar hydatid cyst sections from AE model mice treated with IgG (n=3), P29 mAb (n=3), or P29 mAb kbhb (n=3). Three mice were randomly selected from each group (n=9) for analysis, with three microscopic fields quantified per sample. All data are presented as mean ± SEM. Statistical significance was determined by two-tailed Student’s t-test, with exact p-values indicated directly on the figure. PSC, Protoscolex. HE, hematoxylin and eosin; PAS, Periodic Acid-Schiff; GL, germinal layer; LL, laminated layer; PV, parasitic vesicle.

Article Snippet: PAS staining was conducted strictly according to the manufacturer’s protocol of PAS staining kit (G1008, Servicebio, China).

Techniques: Drug discovery, Infection, Staining, TUNEL Assay, Imaging, Fluorescence, Two Tailed Test

Journal: International Journal of Oral Science

Article Title: Cuproptosis promotes inflammatory osteolysis via GYS1-mediated glycogen metabolism

doi: 10.1038/s41368-025-00408-1

Figure Lengend Snippet: Glycogen synthesis is inhibited in cuproptosis. a Metabolic pathway enrichment analysis of metabolites in macrophages after ES-Cu treatment. b Volcano plot of differentially presented metabolites after ES-Cu treatment. c Diagram of glycolysis, pentose phosphate pathway (PPP), glycogen metabolism (GM). d Gys1 mRNA levels in macrophages. e Western blot analysis of PGM1, GYS1, UGP2 and PYGL expression in ES-Cu treated macrophages. f G1P levels in ES-Cu-treated macrophages were detected by LC-MS. PAS staining ( g ) and colorimetric assay ( h ) were used to detect the glycogen levels in macrophages. Scale bar: 50 μm. Data are from three independent experiments. All error bars are mean ± SEM. P values were calculated by 1-way ANOVA followed by Tukey’s multiple-comparison test

Article Snippet: PAS staining was utilized to detect glycogen in tissues and cells by a PAS Assay Kit (Servicebio, G1008).

Techniques: Western Blot, Expressing, Liquid Chromatography with Mass Spectroscopy, Staining, Colorimetric Assay, Comparison